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KINETICS OF AMINO ACID INFLUX INTO NITELLA FLEXILIS 1
Author(s) -
Wallen D. G.
Publication year - 1973
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1973.tb04070.x
Subject(s) - biology , glycine , nitella , amino acid , alanine , kinetics , valine , aspartic acid , biophysics , botany , biochemistry , physics , quantum mechanics
SUMMARY The uptake of amino acids by Nitella flexilis has been investigated. Influx of glycine, alanine, and valine appears to be a diffusive process. Influx ranged from 0.14 to 0.06 and 0.04 pmoles/(cm)(sec), respectively. Aspartic acid uptake is an active transport mechanism. The V max is 2.8 pmoles/(cm)(sec); the transport constant (Michaelis constant) K m , 7.8 × 10 −3 M. The uptake of arginine is apparently due to 2 transport systems, one with a V max and K m of 3.1 pmoles/(cm)(sec) and 3.2 × 10 −3 M, respectively. The second system has a V max of 1.4 pmoles/(cm)(sec) and a K m of 2.1 × 10 −4 M. The possibility that the second system is diffusive has been considered.