Premium
THE ANTIBACTERIAL SUBSTANCES OF THE MARINE ALGA STICHOCHRYSIS IMMOBILIS (CHRYSOPHYTA) 1 2
Author(s) -
Berland Berigitte R.,
Bonin Daniel J.,
Cornu Alain L.,
Maestrini Serge Y.,
Marino JeanPaul
Publication year - 1972
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1972.tb04052.x
Subject(s) - sephadex , biology , size exclusion chromatography , bacteria , amino acid , algae , thin layer chromatography , peptide , marine bacteriophage , antibacterial activity , chemical structure , chromatography , biochemistry , microbiology and biotechnology , nuclear chemistry , botany , organic chemistry , chemistry , enzyme , genetics
SUMMARY Two substances with antibacterial activity have been concentrated from the culture medium of Stichochrysis immobilis before senescence occurred, and purified, by a Sephadex G‐25 filtration followed by 2‐dimensional thin‐layer chromatography on Merck F‐254 silica gel plates. One of the substances is obviously a peptide; 11 different amino acids are linked in a 30 amino acid molecule (MW 3400). It was not possible to assign a chemical group to the second substance (MW ≃ 2600); it may be related to “humic acids” (Kalle's gelbstoff). Growth of the most sensitive bacteria was completely inhibited by 20‐fold, or higher, concentrations of substances from the algal culture filtrate (assuming no loss of substances during the concentration processes). The 54 strains of test bacteria were of various origins, though mostly isolated from algal cultures or obtained directly from the marine environment.