Premium
RESULTS OF AN ATTEMPT TO OBTAIN PYRENOIDS OF ZYGNEMA BY BULK‐ISOLATION METHODS 1 , 2
Author(s) -
Rosowski James R.,
Hoshaw Robert W.
Publication year - 1971
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1971.tb01525.x
Subject(s) - pyrenoid , biology , sucrose , filtration (mathematics) , chromatography , botany , biophysics , chloroplast , biochemistry , chemistry , statistics , mathematics , gene
SUMMARY Filaments of Zygnema were blended 3 min in a buffered sucrose solution, and a pyrenoid‐rich cell fraction was obtained by filtration through a 10‐μ filter. The filtrate was placed on a 35%/40%/50% (wt/wt) discontinuous sucrose density gradient and was spun at approximately 50,000 × g for 1/2–1 hr. Pyrenoids, which were collected at the 40%/50% interface, appeared with light microscopy to be firm, intact, spherical, colorless refractive bodies. Although in situ pyrenoids stained with propionocarmine, isolated pyrenoids would not stain, indicating a change in the chemical properties of pyrenoids during the isolation procedure.