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STIMULATORY PROPERTIES OF FILTRATE FROM THE GREEN ALGA HORMOTILA BLENNISTA. II. FRACTIONATION OF FILTRATE 1 , 2
Author(s) -
Monahan Thomas J.,
Trainor Francis R.
Publication year - 1971
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1971.tb01497.x
Subject(s) - fractionation , biology , extracellular , chromatography , population , biochemistry , food science , chemistry , demography , sociology
SUMMARY This investigation reports on the fractionation of filtrate from the green alga Hormotila blennista known to contain autostimulatory properties. Acid, basic, and volatile acid filtrate extracts reduced the lag time of H. blennista at low concentrations. Whole filtrate did not express those lag time reducing capacities which were demonstrated in filtrate extracts. Glycolic acid was identified in both the acid and volatile acid extracts. Growth rate stimulation could not be demonstrated with any filtrate extract. Stimulatory properties of filtrate were shown to be dialyzable and heat labile. It was suggested that heat‐labile low molecular weight organic extracellular products are responsible for the growth rate stimulatory property of filtrate. Although dialysis and heat treatments of filtrate removed growth rate stimulation, filtrate properties capable of extending final population levels were retained. High molecular weight heat‐stable extracellular products appear to be at least partially responsible for these extended growth levels.