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DIVISION SYNCHRONY IN PRIMARY CULTURES OF AN ENDOZOIC DINOFLAGELLATE 1
Author(s) -
Franker Colin K.
Publication year - 1971
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1971.tb01496.x
Subject(s) - biology , cell division , dinoflagellate , division (mathematics) , cell cycle , zooxanthellae , thymidine , dna synthesis , dna , botany , microbiology and biotechnology , genetics , cell , bacteria , arithmetic , mathematics , symbiosis
SUMMARY Division cysts of zooxanthellae harbored by Anthopleura elegantissima were isolated from random host‐associated populations by their preferential attachment to plastic surfaces. By selecting cysts at similar stages in the cell cycle, synchronous division, excystation, and daughter cell reencystment were obtained in a medium enriched with amino acids. The interval of DNA synthesis in a single growth cycle was determined by pulse‐labeling with radioactive thymidine. Analysis of the sequence of morphologic changes within such cycles suggested that the initial selection procedure isolates G 2 cells. The transit time from first generation G 2 division cysts to subsequent reentry into G 2 was about 70 hr.