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FREEZE‐DRYING OF BLUE‐GREEN ALGAE FOR ELECTRON MICROSCOPE STUDIES1
Author(s) -
Rogers Tom D.,
Scholes Ver E.,
Schlichting Harold E.
Publication year - 1967
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1967.tb04652.x
Subject(s) - biology , electron microscope , freeze drying , capsule , botany , ultrastructure , thylakoid , chloroplast , chromatography , biochemistry , chemistry , physics , gene , optics
SUMMARY Cultures of Anabaena cylindrica Lemm. and Eucapsis sp. were harvested, freeze‐dried, and prepared for electron microscope studies. A comparison of the freeze‐dried preparations was made with control specimens not freeze‐dried. Fixation and embedding were according to the Ryter and Kellenberger technique. The photosynthelic thylakoids of the freeze‐dried cells were more distinct and less distorted than those of the control cells. Freeze‐dried Eucapsis sp. cells exhibited a prominent mucous capsule, whereas in the nonfreeze‐dried, no such capsule could be discerned.