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FURTHER OBSERVATIONS ON GROWTH OF SINGLE CELLS OF COCCOID BLUE‐GREEN ALGAE
Author(s) -
Baalen Chase
Publication year - 1967
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1967.tb04650.x
Subject(s) - biology , algae , serial dilution , growth medium , strain (injury) , lag time , lag , biophysics , microbiology and biotechnology , botany , bacteria , anatomy , biological system , medicine , computer network , alternative medicine , genetics , pathology , computer science
SUMMARY Initiation of growth (lag) and subsequent growth to visible colonies was examined for single cells of several coccoid blue‐nreen algae under controlled incuba‐tion conditions. At 30 or 39 C, with tungsten or fluorescent illumination, organisms such as Agmenel‐lum quadruplicatum, strains PR‐6 and strain BG‐J, showed no evidence of lag associated with initiation of growth. The final colony count was within 20% of the expected number derived from cell counts and serial dilutions.For Anacystis nidulnns, Tx 20, a new medium C, 10 with EDTA as chelator was developed. In this medium groiuth of single cells at 39 C was excellent with quantitative recovery and no evidence of lag. At 30 C, however, Tx 20 showed anomalous behavior, lag and nonquantitative cell recovery. This behavior at 30 C is not yet understood.