Premium
Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system
Author(s) -
Gregson Norman A.,
Zhang Gaiping,
Pritchard Jane,
Wang Aiping,
Sanvito Lara,
Hayday Adrian C.,
Hughes Richard A. C.
Publication year - 2007
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1111/j.1529-8027.2007.00112.x
Subject(s) - monoclonal antibody , myelin , peripheral myelin protein 22 , peripheral nervous system , biology , immunohistochemistry , antibody , central nervous system , fetus , pathology , microbiology and biotechnology , nervous system , immunology , medicine , neuroscience , pregnancy , genetics
We produced a mouse monoclonal antibody using cDNA and peptide immunization against the putative second extra‐cellular domain of human peripheral myelin protein 22 (PMP22). It reacted specifically with human PMP22 and not with other human myelin proteins and did not react with bovine, rat, or mouse PMP22. The antibody stained the compact myelin of human peripheral nerve motor and sensory axons and did not stain central nervous system tissue. PMP22 reactivity was detected in the spinal roots of the human fetus at 19–20 weeks of gestation. The staining pattern of the PMP22 antibody resembled that of a monoclonal antibody directed against the myelin protein zero.