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Distribution and proliferation of bone marrow cells in the brain after pilocarpine‐induced status epilepticus in mice
Author(s) -
Longo Beatriz,
Romariz Simone,
Blanco Miriam Marcela,
Vasconcelos Juliana Fraga,
Bahia Luciana,
Soares Milena Botelho Pereira,
Mello Luiz E.,
RibeirodosSantos Ricardo
Publication year - 2010
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/j.1528-1167.2010.02570.x
Subject(s) - status epilepticus , pilocarpine , bone marrow , microglia , green fluorescent protein , hippocampus , distribution (mathematics) , genetically modified mouse , pathology , transgene , biology , chemistry , medicine , endocrinology , microbiology and biotechnology , immunology , neuroscience , epilepsy , inflammation , biochemistry , mathematical analysis , mathematics , gene
Summary The distribution of bone marrow cells in brain areas during the acute period after pilocarpine‐induced status epilepticus (SE) was investigated here. To achieve this, we generated chimeric mice by engrafting bone marrow cells from enhanced green fluorescent protein (eGFP) transgenic mice. GFP + bone marrow–derived cells were found throughout the brain, predominantly in the hippocampus. As expected, these cells exhibited the characteristics of microglia. The pattern of distribution, proliferation, and differentiation of GFP + cells changes as a function of intensity and time following SE. This pattern is also a consequence of the inflammatory response, which is followed by the progressive neuronal damage that is characteristic of the pilocarpine model.