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A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC‐tandem mass spectrometry
Author(s) -
La Marca Giancarlo,
Malvagia Sabrina,
Filippi Luca,
Luceri Francesca,
Moneti Gloriano,
Guerrini Renzo
Publication year - 2009
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/j.1528-1167.2009.02204.x
Subject(s) - chromatography , phenobarbital , chemistry , dried blood spot , spots , dried blood , mass spectrometry , tandem mass spectrometry , coefficient of variation , newborn screening , immunoassay , liquid chromatography–mass spectrometry , calibration curve , analytical chemistry (journal) , detection limit , medicine , pharmacology , biochemistry , immunology , antibody
Summary Advantages of dried blood spot include low invasiveness, ease and low cost of sample collection, transport, and storage. We used tandem mass spectrometry (LC‐MS/MS) to determine phenobarbital levels on dried blood spot specimens and compared this methodology to commercially available particle enhanced turbidimetric inhibition immunoassay (PETINIA) in plasma/serum samples. The calibration curve in matrix using D 5 ‐phenobarbital as internal standard was linear in the phenobarbital concentration range of 1–100 mg/L (correlation coefficient 0.9996). The coefficients of variation in blood spots ranged 2.29–6.71% and the accuracy ranged 96.54–103.87%. There were no significant differences between the concentrations measured using PETINA and LC‐MS/MS (both had similar precision and accuracy) however, LC‐MS/MS allows at least 1.5 times higher throughput of phenobarbital analysis and additionally offers ease of sample collection which is particularly important for newborns or small infants.