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Effects of Dibutyryl Cyclic AMP on Na + , K + ‐ATPase Activity and Intracellular Na + and K + in Primary Cultures of Astrocytes from DBA and C57 Mice
Author(s) -
Li Jun,
Chow SienYao
Publication year - 1994
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/j.1528-1157.1994.tb02907.x
Subject(s) - intracellular , atpase , neuroglia , endocrinology , cell culture , astrocyte , medicine , microbiology and biotechnology , biology , chemistry , biochemistry , enzyme , central nervous system , genetics
Summary: Effects of chronic treatment of dibutyryl cyclic AMP (db‐cyclic AMP) on Na + , K + ‐ATPase activity in cell homogenates and intracellular N a f and K+ contents [(Na + ) i and (K + ) i ] were studied in primary cultures of astrocytes derived from cerebral cortex of neonatal audiogenic seizure‐susceptible DBA and audiogenic seizure‐resistant C57 mice. Na + , K + ‐ATPase activity in cell homogenates was greater and (Na + ) i was less in DBA astrocytes than in C57 astrocytes. There was no difference in (K + ) i between astrocytes from DBA and C57 mice. Addition of db‐cyclic AMP to the medium from day 14 to day 21 in culture (final concentration 0.25 m M ) increased Na + , K + ‐ATPase activity in cell homogenates and decreased (Na + ) i , but had no significant effect on (K + ) i in astrocytes from either DBA or C57 mice. Chronic treatment with db‐cyclic AMP altered cell growth. Protein and DNA content of cultured astrocytes from both DBA and C57 mice was decreased. DNA was more affected than protein. Modifying K + and Na + concentration in medium altered Na + , K + ‐ATPase activity in cell homogenates as well as (Na + ) i and (K + ) i in cultured astrocytes of both DBA and C57 mice. Changes in (Na + ) i and (K + ) i at different K + concentrations in medium paralleled those in Na + , K + ‐ATPase activity in cell homogenates. Results indicate that the ability to transport Na + across the cell membrane and the response of Na + , K + ‐ATPase to db‐cyclic AMP and to the changes in K + in medium of cultured astrocytes from audiogenic seizure‐susceptible DBA mice are sufficient.