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Effect of Kainic Acid on Unit Discharge in CA1 Area of Hippocampal Slice of DBA and C57 Mice
Author(s) -
Wang Zhian,
Chow SienYao
Publication year - 1994
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/j.1528-1157.1994.tb02534.x
Subject(s) - kainic acid , hippocampal formation , population , epilepsy , chemistry , cerebrospinal fluid , perfusion , hippocampus , anesthesia , medicine , endocrinology , neuroscience , biology , glutamate receptor , receptor , environmental health
Summary: Spontaneous unit discharges in stratum pyramidale of CA1 area of hippocampal slices from DBA and CS7 mice at different ages were recorded extracellularly. The average rate and amplitude of the spontaneous discharges from CA1 area of hippocampal slices bathed in artificial cerebrospinal fluid (aCSF) were not different between DBA and CS7 mice at either 3–4 or 5–6 weeks of age. Bath application of kainic acid (KA) in concentrations of 0·5–1·0 μM produced different responses in CA1 area from these two strains of mice. In DBA mice at age 3–4 weeks, when they are most susceptible to audiogenic seizures, KA perfusion induced high‐frequency repetitive single spikes and bursts of multiple population spikes in CA1 area. Very high‐frequency discharges (10‐fold higher than most responses) were also observed in 20% of all slices. In audiogenic seizure resistant CS7 mice at age 3–4 weeks, KA perfusion at the same doses induced only the repetitive single spikes. The rate of spontaneous discharges was much lower than that in DBA mice. No burst of multiple population spikes nor very high‐frequency responses were recorded in CS7 mice. At age 5–6 weeks, when both DBA and CS7 mice are resistant to audiogenic seizures, the rate of spontaneous discharges recorded from the CAI area during and after KA perfusion was lower than that at age 3–4 weeks, and there was no significant difference between DBA and CS7 mice. Pretreatment of hippocampal slices with AMPA/kainate receptor antagonist CNQX (10 pA4 ) markedly reduced the rate and amplitude of spontaneous discharges in CA1 area during and after KA perfusion, whereas competitive N‐methyl‐D‐aspartate (NMDA) receptor antagonist D‐APS had no effect. These results indicate that the responses in spontaneous discharges recorded extracellularly from stratum pyramidale in CA1 area of hippocampal slices to KA perfusion correlate with susceptibility to audiogenic seizures in DBA and CS7 mice.