Open AccessMineralocorticoid Receptor Is Overexpressed in Cardiomyocytes of Patients With Congestive Heart Failure
Author(s) -
Yoshida Masahiro,
Ma Jun,
Tomita Tsutomu,
Morikawa Norihiro,
Tanaka Nobuyoshi,
Masamura Katsuhiko,
Kawai Yasuyuki,
Miyamori Isamu
Publication year - 2005
Publication title -
congestive heart failure
Language(s) - English
Resource type - Journals
eISSN - 1751-7133
pISSN - 1527-5299
DOI - 10.1111/j.1527-5299.2005.03722.x
Subject(s) - heart failure , ventricle , mineralocorticoid receptor , medicine , in situ hybridization , aldosterone , mineralocorticoid , immunohistochemistry , endocrinology , receptor , cytoplasm , cardiology , staining , pathology , messenger rna , biology , microbiology and biotechnology , biochemistry , gene
Mineralocorticoid receptors (MRs) have been identified in the human cardiovascular tissues. We determined MR expression in the failing heart to clarify the mechanism of action of aldosterone antagonist in the treatment of congestive heart failure. MR protein and MR mRNA content were detected by immunohistochemical staining and in situ hybridization in the cardiac tissues. Immunohistochemical staining of the receptor, as well as in situ hybridization of MR mRNA, was dense in cardiomyocytes of the failing left ventricle as compared with the controls. The staining ratio of the cytoplasm to the interstitium showed that MRs were located mainly in the cytoplasm. The cytoplasm to the interstitium in the failing left ventricle was 1.53±0.13, which was significantly higher than that of the controls 1.25±0.19 ( p <0.05). These findings suggest that the efficacy of aldosterone antagonists in treating congestive heart failure may be in part through blocking the MRs, which are upregulated in the failing heart.