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Lack of Association of Endothelial Nitric Oxide Synthase Polymorphisms and Migraine
Author(s) -
Toriello María,
Oterino Agustín,
Pascual Julio,
Castillo Jesús,
Colás Rafael,
AlonsoArranz Ana,
RuizAlegría Carlos,
Quintela Estrella,
Montón Fernando,
RuizLavilla Nuria
Publication year - 2008
Publication title -
headache: the journal of head and face pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.14
H-Index - 119
eISSN - 1526-4610
pISSN - 0017-8748
DOI - 10.1111/j.1526-4610.2008.01181.x
Subject(s) - enos , migraine , aura , haplotype , genotype , allele , nitric oxide , migraine with aura , linkage disequilibrium , medicine , nitric oxide synthase , allele frequency , genetics , endocrinology , biology , gene
Objective.— The aim of this study was to evaluate if 2 functional endothelial nitric oxide synthase (eNOS) gene polymorphisms might be risk factors for migraine. Background.— Nitric oxide synthase promotes the synthesis of nitric oxide (NO). NO is a potent vasodilator and mediates several processes involved in migraine pathophysiology. Only one study has suggested an association with migraine with aura. Methods.— We performed a sex‐ and age‐matched case‐control study using 2 eNOS polymorphisms (rs1800779 and rs1799983), which are in linkage disequilibrium. Genotypes were obtained with allele‐specific probes in a real‐time polymerase chain reaction assay. Genotypic and allelic distributions were compared with χ 2 method. We also estimated the reconstructed haplotypes and calculated ORs for individual haplotypes. Results.— A total of 337 migraine patients (188 with aura) and 341 healthy controls were recruited. We found no significant differences in the distribution of genotypes and alleles for either polymorphism among clinical subgroups. Neither rs1800779 nor rs1799983 polymorphisms increased the risk for suffering from migraine aura. Conclusions.— As others have previously reported, we failed to demonstrate genetic association of the eNOS gene with migraine.