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Investigation of Some Biological Trait Markers in Migraine: Deuterated Tyramine Challenge Test, Monoamine Oxidase, Phenolsulfotransferase and Plasma and Urinary Biogenic Amine and Acid Metabolite Levels
Author(s) -
Davis B.A.,
Dawson B.,
Boulton A.A.,
Yu P.H.,
Durden D.A.
Publication year - 1987
Publication title -
headache: the journal of head and face pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.14
H-Index - 119
eISSN - 1526-4610
pISSN - 0017-8748
DOI - 10.1111/j.1526-4610.1987.hed2707384.x
Subject(s) - tyramine , chemistry , monoamine oxidase , metabolite , biogenic amine , urine , phenylacetic acid , substrate (aquarium) , urinary system , chromatography , biochemistry , endocrinology , medicine , serotonin , enzyme , biology , receptor , ecology
SYNOPSIS A search for biological “trait” markers in migraine patients was undertaken. Migraine and control subjects were challenged with an oral dose of deuterated r ‐tyramine. Deuterium‐labelled conjugated and unconjugated r ‐tyramine and r ‐hydroxyphenylacetic acid in 24 hour urine samples, as well as several endogenous biogenic amines and their acidic metabolites in blood and urine, were identified and quantitated by mass spectrometry with selected ion monitoring. In contrast to earlier reports, urinary conjugated r ‐tyramine was not significantly reduced in migraine. Platelet monoamine oxidase (with r ‐tyramine as substrate) and phenolsulfotransferase (with phenol as substrate) activities were significantly reduced. Plasma unconjugated vanilmandelic acid and conjugated phenylacetic acid and urinary unconjugated 3‐methoxy‐4‐hydroxyphenylethylene glycol were significantly elevated in migraine. These parameters would not, however, be suitable as biological markers because the differences compared to controls did not attain a sufficiently high level of statistical significance.