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Leukotriene B 4 Generation by Polymorphonuclear Leukocytes: Possible Involvement in the Pathogenesis of Headache
Author(s) -
Selmaj K.,
Belleroche J.,
Das I.,
Rose F. Clifford
Publication year - 1986
Publication title -
headache: the journal of head and face pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.14
H-Index - 119
eISSN - 1526-4610
pISSN - 0017-8748
DOI - 10.1111/j.1526-4610.1986.hed2609460.x
Subject(s) - cluster headache , migraine , stimulation , leukotriene , pathogenesis , leukotriene b4 , medicine , immunology , endocrinology , inflammation , asthma
SYNOPSIS Leukotriene B 4 (LTB 4 ) levels in plasma and its generation in vitro from isolated polymorphonuclearleukocytes (PMN) were measured in migraine and cluster headache patients during and between painattacks. The LTB 4 plasma levels in cluster headache patients during an attack were significantly higherthan in patients between attacks. There was a positive correlation between the time of sampling from thebeginning of attack and the LTB 4 level. The LTB 4 plasma levels in migraine patients during and betweenattacks did not differ significantly from control levels. The results suggest that LTB 4 appears rapidly in thecirculation at the beginning of a cluster attack and thereafter declines in amount. LTB 4 release from PMNwas induced by stimulation with the calcium ionophore A23187. The release of LTB 4 was significantlyhigher in migraine patients during attacks on stimulation with a submaximal dose of A23187 0.5 μM. LTB 4 release induced by A23187 in migraine patients during symptom‐free intervals and cluster headachepatients did not differ from healthy controls. LTB 4 release was not affected by 5HT and NA inconcentrations up to 10 ‐4 M. The results suggest that LTB 4 is more easily generated in migraine patientsduring attacks. These studies on isolated cells provide a suitable model for the investigation of themetabolism of lipoxygenase derivates in headache patients.