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Endothelin‐1 Acutely Reduces the Permeability of Visceral Sheep Peritoneum In Vitro Through Both Endothelin‐ A and Endothelin‐ B Receptors
Author(s) -
Kourti Panagiota,
Zarogiannis Sotirios G.,
Liakopoulos Vassilios,
Karioti Aggeliki,
Eleftheriadis Theodoros,
Hatzoglou Chrissi,
Gourgoulianis Konstantinos,
Molyvdas PaschalisAdam,
Stefanidis Ioannis
Publication year - 2013
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2012.01565.x
Subject(s) - peritoneum , endothelin receptor , receptor , mesothelium , antagonist , endocrinology , medicine , chemistry , in vitro , endothelin 1 , pharmacology , anatomy , biochemistry
Mesothelium is an important part of the peritoneal barrier for water and ion transport, essential for effective peritoneal dialysis ( PD ). Peritoneal fibrosis has been associated with PD treatment failure. Endothelin‐1 ( ET ‐1) is a potent vasoactive peptide, involved in pathologic fibrotic processes. Its action is mediated mainly by endothelin type A ( ET A ) and type B ( ET B ) receptors. The aim of this study was to investigate, by U ssing chamber experiments, the effect of ET ‐1 on the transmesothelial electrical resistance ( R TM ) of the isolated visceral sheep peritoneum. Intact sheets of visceral peritoneum were obtained from 40 adult sheep and mounted in Ussing‐type chambers. ET ‐1 (10 −7 M), BQ ‐123 ( ET A receptor antagonist; 10 −6 M ), BQ ‐788 ( ET B receptor antagonist; 10 −6 M ), and their combinations were added on the apical and the basolateral side of the peritoneum. R TM was measured before and serially after addition of the substances, and changes were registered as percentage (Δ R TM %). R TM increased within 1 min after addition of ET ‐1 apically (Δ R TM 65.03 ± 15.87%; P < 0.05) or basolaterally (Δ R TM 85.5 ± 20.86%; P < 0.05). BQ ‐123 and BQ ‐788 and their combination significantly reduced ( P < 0.05) the effect of ET ‐1 to a similar degree in all cases. These results clearly indicate that ET ‐1 reduces ionic permeability of the visceral sheep peritoneum in vitro. Additionally, it is obvious that this inhibitory effect is mediated through both ET A and ET B receptors.