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Three‐Dimensional Geometry of Honeycomb Collagen Promotes Higher Beating Rate of Myocardial Cells in Culture
Author(s) -
Guo Zhikun,
Iku Shouhei,
Zheng Xianjie,
Sammons Rachel L.,
Kuboki Yoshinori
Publication year - 2012
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2012.01446.x
Subject(s) - scaffold , regeneration (biology) , tissue engineering , honeycomb , matrix (chemical analysis) , biomedical engineering , chemistry , biophysics , materials science , composite material , microbiology and biotechnology , biology , medicine
Myocardial cells were isolated from newborn rats, cultured on a novel three‐dimensional (3‐D) honeycomb collagen scaffold (HC) and their morphology and beating rates compared with ones on conventional plastic dishes. On the first day, the cells attached to HC had already started beating. As time went on, the rate of beating increased as the pores of HC gradually filled with the cells, which integrated to form the cell–matrix complex. At day 8, beating reached the highest frequency of 162 beats per minute, which was twice that of the control cells on plastic dishes. It was concluded that 3‐D geometry of the HC is conducive to functional growth of the myocardial tissues, and will potentially be useful for tissue engineering of myocardial regeneration.