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Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development
Author(s) -
Duailibi Monica Talarico,
Duailibi Silvio Eduardo,
Duailibi Neto Eduardo Felippe,
Negreiros Renata Matalon,
Jorge Waldyr Antonio,
Ferreira Lydia Masako,
Vacanti Joseph Phillip,
Yelick Pamela Crotty
Publication year - 2011
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2010.01200.x
Subject(s) - tissue engineering , molar , dentistry , human tooth , dental pulp stem cells , pulp (tooth) , hard tissue , developmental stage , stem cell , orthodontics , biomedical engineering , medicine , biology , microbiology and biotechnology , enamel paint , psychology , developmental psychology
Our long‐term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony‐forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.