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Effects of Spermidine and p‐Cresol on Polymorphonuclear Cell Apoptosis and Function
Author(s) -
de Carvalho Jr Jose Tarcisio G.,
Dalboni Maria A.,
Watanabe Renato,
Peres Alines T.,
Goes Miguel A.,
Manfredi Silvia R.,
Canziani Maria E.,
Cendoroglo Gabriel S.,
GuimarãesSouza Nadia,
Batista Marcelo C.,
Cendoroglo Miguel
Publication year - 2011
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2010.01116.x
Subject(s) - spermidine , apoptosis , viability assay , chemistry , microbiology and biotechnology , medicine , andrology , pharmacology , biology , endocrinology , biochemistry , enzyme
Polymorphonuclear leukocytes (PMNs) from chronic kidney disease (CKD) patients display accelerated apoptosis and dysfunction, which may predispose CKD patients to infections. In this study, we investigated the effect of spermidine and p‐cresol on apoptosis and function on PMN from healthy subjects. We measured the effect of spermidine and p‐cresol on apoptosis, ROS production unstimulated and stimulated ( S. aureus and PMA) and expression of CD95, caspase 3, and CD11b on PMN. After incubation with p‐cresol and spermidine, we did not observe any changes in apoptosis, viability or expression of caspase 3 and CD95 in PMN from healthy subjects. PMN incubated for 10 minutes with spermidine demonstrated a significant reduction in spontaneous, S. aureus and PMA‐stimulated ROS production. p‐cresol induced a decrease in PMA‐stimulated ROS production. Spermidine and p‐cresol also induced a decrease in the expression of CD11b on PMN. Spermidine and p‐cresol decreased the expression of CD11b and oxidative burst of PMN from healthy subjects and had no effect on PMN apoptosis and viability.