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Bone Marrow Combined With Dental Bud Cells Promotes Tooth Regeneration in Miniature Pig Model
Author(s) -
Kuo TzongFu,
Lin HsinChi,
Yang KaiChiang,
Lin FengHuei,
Chen MinHuey,
Wu ChangChin,
Chang HaoHueng
Publication year - 2011
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2010.01064.x
Subject(s) - cementum , dentin , periodontal fiber , regeneration (biology) , bone marrow , scaffold , dental alveolus , miniature swine , chemistry , dentinogenesis , biomedical engineering , odontoblast , anatomy , microbiology and biotechnology , dentistry , pathology , biology , medicine
Growth factors and morphogens secreted by bone marrow mesenchymal stem cells (BMSCs) of bone marrow fluid may promote tooth regeneration. Accordingly, a tissue engineering approach was utilized to develop an economical strategy for obtaining the growth factors and morphogens from BMSCs. Unerupted second molar tooth buds harvested from miniature pigs were cultured in vitro to obtain dental bud cells (DBCs). Bone marrow fluid, which contains BMSCs, was collected from the porcine mandible before operation. DBCs suspended in bone marrow fluid were seeded into a gelatin/chondoitin‐6‐sulfate/hyaluronan tri‐copolymer scaffold (GCHT scaffold). The DBCs/bone marrow fluid/GCHT scaffold was autografted into the original alveolar sockets of the pigs. Radiographic and histological examinations were applied to identify the structure of regenerated tooth at 40 weeks postimplantation. The present results showed that one pig developed a complete tooth with crown, root, pulp, enamel, dentin, odontoblast, cementum, blood vessel, and periodontal ligament in indiscriminate shape. Three animals had an unerupted tooth that expressed dentin matrix protein‐1, vascular endothelial growth factor, and osteopontin; and two other pigs also had dental‐like structure with dentin tubules. This study reveals that DBCs adding bone marrow fluid and a suitable scaffold can promote the tooth regeneration in autogenic cell transplantation.