Premium
Bone Morphogenetic Protein‐6‐loaded Chitosan Scaffolds Enhance the Osteoblastic Characteristics of MC3T3‐E1 Cells
Author(s) -
Akman Abdullah C.,
Seda Tığlı R.,
Gümüşderelioğlu Menemşe,
Nohutcu Rahime M.
Publication year - 2010
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2009.00798.x
Subject(s) - bone morphogenetic protein 2 , chitosan , bone morphogenetic protein 7 , bone morphogenetic protein , chemistry , microbiology and biotechnology , scaffold , biomedical engineering , biochemistry , biology , medicine , in vitro , gene
The purpose of this study is to investigate the convenience of bone morphogenetic protein‐6 (BMP‐6)‐loaded chitosan scaffolds with preosteoblastic cells for bone tissue engineering. MC3T3‐E1 cells were seeded into three different groups: chitosan scaffolds, BMP‐6‐loaded chitosan scaffolds, and chitosan scaffolds with free BMP‐6 in culture medium. Tissue‐engineered constructs were characterized by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazoliumbromide assay, scanning electron microscopy (SEM), mineralization assay (von Kossa), alkaline phosphatase (ALP) activity, and osteocalcin (OCN) assays. BMP‐6‐loaded chitosan scaffolds supported proliferation of the MC3T3‐E1 mouse osteogenic cells in a similar pattern as the unloaded chitosan scaffolds group and as the chitosan scaffolds with free BMP‐6 group. SEM images of the cell‐seeded scaffolds revealed significant acceleration of extracellular matrix synthesis in BMP‐6‐loaded chitosan scaffolds. Both levels of ALP and OCN were higher in BMP‐6‐loaded chitosan scaffold group compared with the other two groups. In addition, BMP‐6‐loaded scaffolds showed strong staining in mineralization assays. These findings suggest that BMP‐6‐loaded chitosan scaffold supports cellular functions of the osteoblastic cells; therefore, this scaffold is considered as a new promising vehicle for bone tissue engineering applications.