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Coatings of Low‐Density Lipoprotein and Synthetic Glycoconjugates as Substrata for Hepatocytes
Author(s) -
Yura Hirofumi,
Ishihara Masayuki,
Nakamura Shingo,
Kishimoto Satoko,
Kanatani Yasuhiro,
Horio Takuya,
Ishizuka Takamitsu,
Kawakami Mitsuyuki,
Matsui Takemi
Publication year - 2009
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2009.00746.x
Subject(s) - asialoglycoprotein receptor , glycoconjugate , endocytosis , biochemistry , chemistry , ldl receptor , fetuin , low density lipoprotein , glycoprotein , lectin , extracellular matrix , galactose , lactate dehydrogenase , receptor , lipoprotein , hepatocyte , cholesterol , in vitro , enzyme
Asialoglycoprotein (ASGP) receptors expressed on rat hepatocytes interact with glycoproteins containing galactose or N‐acetylgalactosamine residues at the nonreducing termini of oligosaccharide chains to mediate endocytosis, and cholesterol transport protein with apolipoprotein B (LDL, low‐density lipoprotein) in plasma interacts with LDL receptors and heparinoids in the extracellular matrix. We developed novel techniques to prepare galactose‐ and LDL‐immobilized culture plates, using galactose‐tagged polystyrene (galactose‐carrying polystyrene [GalCPS]: N‐p‐vinylbenzyl‐O‐β‐D‐galactopyranosyl‐[1→4]‐D‐gluconamide) and poly(2‐acrylamide‐2‐methyl‐1‐propanesulfonate) (PAPS), respectively. Hepatocytes adhered well to plates coated with either GalCPS or LDL, and therefore the GalCPS‐ and LDL‐coated plates were examined as specific substrata for culturing hepatocytes. These cultures promoted the formation of three‐dimensional, multicellular aggregates with regulation of excess proliferation of non‐parenchymal cells. Furthermore, the LDL coating resulted in higher albumin synthesis and an identical level of lactate dehydrogenase (LDH) compared with cells cultured on collagen‐ and GalCPS‐coated plates. Thus, the two culture systems described here, and especially the LDL‐coated plates, have potential for the development of a hybrid artificial liver.