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Fragmin/Protamine Microparticle‐Coated Matrix Immobilized Cytokines to Stimulate Various Cell Proliferations With Low Serum Media
Author(s) -
Kishimoto Satoko,
Nakamura Shingo,
Nakamura Shinichiro,
Kanatani Yasuhiro,
Hattori Hidemi,
Tanaka Yoshihiro,
Harada Yasuji,
Tagawa Masahiro,
Mori Yasutaka,
Maehara Tadaaki,
Ishihara Masayuki
Publication year - 2009
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2009.00745.x
Subject(s) - fetal bovine serum , basic fibroblast growth factor , cytokine , chemistry , matrix (chemical analysis) , in vitro , growth factor , protamine , cell culture , haematopoiesis , fibroblast growth factor , proinflammatory cytokine , heparin , andrology , immunology , microbiology and biotechnology , medicine , biology , stem cell , biochemistry , inflammation , receptor , chromatography , genetics
Fragmin/protamine microparticles (F/P MPs) have been shown to bind to culture plates, thereby retaining heparin‐binding cytokines. Most protocols for in vitro cultures of human microvascular endothelial cells (hMVECs), human dermal fibroblast cells (hDFCs), and hematopoietic cell line (TF‐1) include high fetal bovine serum (FBS) (10%) medium as a nutritional supplement. Growth rates of those cells on the F/P MP‐coated plates were higher in low FBS (1%) medium containing fibroblast growth factor (FGF)‐2 (for hMVECs and hDFCs) and interleukin (IL)‐3/granulocyte‐macrophage colony‐stimulating factor (for TF‐1 cells) than without coating. The cytokines in low FBS medium were shown to be immobilized on the F/P MP‐coated plate and released into the culture medium with a half releasing time of 4–5 days. Furthermore, those cells grew well on each cytokine‐preimmobilized F/P MP‐coated plate in low FBS medium. Thus, the F/P MP‐coated matrix with adequate heparin‐binding cytokines may provide biomaterials for controlling cellular growth and differentiation.