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Real‐time Polymerase Chain Reaction to Follow the Response of Muscle to Training
Author(s) -
Moore Lauren M.,
Fisher Andrew G.,
Coulson Judy M.,
Salmons Stanley,
Jarvis Jonathan C.
Publication year - 2008
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2008.00613.x
Subject(s) - polymerase chain reaction , chain (unit) , real time polymerase chain reaction , training (meteorology) , chain reaction , computer science , chemistry , biochemistry , gene , physics , photochemistry , astronomy , meteorology
The adaptive response of muscle to changes in activity or loading can take many weeks. Changes in the levels of RNA within a muscle fiber can give an early indication of the nature of the response of that fiber to changes in activity or loading. We have designed a new primer set for quantitative polymerase chain reaction (PCR) that will allow us to follow these early transcriptional changes in rat muscle, and have shown that analysis can be performed by standard techniques on as little as 5 mg of muscle, an amount that can be obtained by needle biopsy.