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Oxygen Reduces Accumulation of Type IV Collagen in Endothelial Cell Subcellular Matrix via Oxidative Stress
Author(s) -
Brevig Thomas,
Røhrmann Jette H.,
Riemann Holger
Publication year - 2006
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2006.00324.x
Subject(s) - oxidative stress , oxygen , chemistry , matrix (chemical analysis) , oxidative phosphorylation , reactive oxygen species , cell , endothelial stem cell , extracellular matrix , microbiology and biotechnology , biophysics , biochemistry , biology , organic chemistry , chromatography , in vitro
Anchorage‐dependent cells in culture attach initially to proteins adsorbed to the culture substrate from the medium, and produce and deposit a subcellular matrix during the course of the cultivation. The aim of this study was to determine whether the concentration of O 2 in the culture atmosphere affects the accumulation of type IV collagen and laminin under human endothelial‐cell monolayers. Enzyme‐linked immunoassays on decellularized polystyrene substrates showed less type IV collagen, but not less laminin, under cells incubated in the standard atmosphere (5% CO 2 in air, i.e., ∼20% O 2 ) compared to an atmosphere of 5% O 2 and 5% CO 2 in N 2 . Type IV collagen accumulation was inhibited via oxidative stress, because the inhibitory effect of 20% O 2 was antagonized by antioxidant ascorbic acid, and mimicked by prooxidant pyrogallol and exogenous H 2 O 2 . Measurements of endogenous H 2 O 2 accumulation demonstrated that endothelial cells partially adapt to the high O 2 concentration. These results may have implications in endothelium modeling in vitro and in engineering of endothelial cell sheets and endothelialized vascular grafts.