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Protein L‐agarose for Adsorption of Autoantibodies: A Potential Tool for Extracorporeal Treatment
Author(s) -
Duarte Isa Santos,
Zollner Ricardo de Lima,
Bueno Sonia Maria Alves
Publication year - 2005
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2005.29053.x
Subject(s) - chemistry , adsorption , agarose , chromatography , langmuir , protein adsorption , langmuir adsorption model , hepes , freundlich equation , albumin , blood proteins , nuclear chemistry , biochemistry , organic chemistry
Abstract: This work investigated the adsorption of autoantibodies such as anti‐SS‐A/Ro, anti‐SS‐B/La, anti‐Sm, and anti‐dsDNA on protein L‐agarose gel. In order to determine better conditions for IgG adsorption on this matrix, some buffer systems were tested. Adsorption data were analyzed using the Langmuir and Langmuir–Freundlich isotherm models. The experimental isotherms were best described by the Langmuir–Freundlich model, which indicated negative and positive cooperativities for binding in the presence of PBS and HEPES buffers, respectively. The K d values for phosphate buffered saline solution (PBS) and hydroxyethylpiperazine ethanesulfonic acid (HEPES) were 2.8 × 10 −7 M and 3.2 × 10 −7 M, respectively, which indicate a high affinity between IgG and the immobilized protein L. The amount of protein adsorbed per amount of protein loaded was high for anti‐Sm (44%) and anti‐dsDNA (46%), but low for anti‐SS‐B/La (9%). The amount of albumin adsorbed was lower than 0.06 mg/mL, which may remove the need for a plasma replacement solution in clinical apheresis.