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Proliferation Rates of HepG2 Cells Encapsulated in Alginate Are Increased in a Microgravity Environment Compared With Static Cultures
Author(s) -
Coward Sam M.,
Selden Clare,
Mantalaris Athanasios,
Hodgson Humphrey J.F.
Publication year - 2005
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2005.29026.x
Subject(s) - albumin , bead , cell growth , cell culture , microbiology and biotechnology , chemistry , liver function , biophysics , biochemistry , biology , materials science , medicine , composite material , genetics
This study investigates the effect of rotary culture compared with static culture on the proliferation, cell viability, synthetic function and detoxificatory capacity of HepG2 cells encapsulated in 1% alginate. Cell viability and alginate bead morphology were maintained in the rotary culture system at day 10, while cell number showed a 4.5‐fold increase compared with static culture. Protein production was increased in rotary cultures with a 4.1‐fold increase in total albumin and a 4.4‐fold increase in α1 antitrypsin levels in rotary compared with static culture at day 10. CYP4501A1/2 activity was maintained between the two culture systems. In conclusion, rotary culture increases proliferation rates leading to improved bead packing and a concomitant increase in total protein synthesis, along with maintenance of detoxificatory capacity. This allows a greater level of hepatic function to be expressed in a given volume, offering clear advantages for the design of liver support systems.