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Synthetic Vascular Prosthesis Impregnated With Genetically Modified Bone Marrow Cells Produced Recombinant Proteins
Author(s) -
KankiHorimoto Sachiko,
Horimoto Hitoshi,
Mieno Shigetoshi,
Kishida Kenji,
Watanabe Fusao,
Furuya Eisuke,
Katsumata Takahiro
Publication year - 2005
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2005.00134.x
Subject(s) - recombinant dna , mesenchymal stem cell , bone marrow , chemistry , biomedical engineering , complementary dna , genetically modified organism , tissue engineering , staining , microbiology and biotechnology , pathology , gene , biology , medicine , biochemistry
The aim of this study is to develop an experimental model of small caliber expanded polytetrafluoroethylene (ePTFE) vascular prostheses that produce recombinant proteins by seeding genetically modified bone marrow mesenchymal stem cells (MSC). Beta‐galactosidase (beta‐gal) cDNA was transduced into rat MSC mediated by an adenovirus vector. The cells were impregnated into the ePTFE vascular prostheses measuring 2 mm in internal diameter and 90 µm in fibril length, followed by 48 h of incubation. The expressions of beta‐gal were determined by X‐gal staining. The luminal surface of the ePTFE vascular prostheses was covered with the MSC expressing beta‐gal. Most of the gene‐transduced MSC spread along the fibers forming colonies. These results suggest that small caliber vascular prostheses, in which the inner surface was seeded by genetically modified MSC, produced recombinant proteins. This may be a preliminary model to autocrine functioning vascular prostheses.