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Research into the Development of a Wearable Bioartificial Kidney with a Continuous Hemofilter and a Bioartificial Tubule Device Using Tubular Epithelial Cells
Author(s) -
Saito Akira
Publication year - 2004
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2004.07323.x
Subject(s) - bioartificial liver device , chemistry , polysulfone , creatinine , urea , membrane , renal function , chromatography , biochemistry , hepatocyte , in vitro
Current hemodialysis treatment is insufficient because of intermittent treatment and loss of tubular function. In order to overcome the loss of tubular function, a bioartificial kidney has been developed consisting of continuous hemofiltration (CHF) with 10 L/day of filtrate and a bioartificial tubule device using proximal tubular epithelial cells and hollow fiber membranes. Ten L/day of CHF enabled plasma levels of urea, creatinine, uric acid and, β 2 ‐microglobulin in eight renal failure patients to be maintained at remarkably low levels. The concept was tested with 6 L (4 mL/min) of 10 L/day (7 mL/min) filtrate regenerated by a bioartificial tubule device and 4 L/day (3 mL/min) replaced by food and drinks. Lewis lung cancer‐porcine kidney 1 (LLC‐PK 1 ) cells with a cell density of 10 7 cells/mL were seeded inside polysulfone hollow fiber modules four times at 1 h intervals while rotating the module 90° each time, and were cultured for 48 h to form confluent monolayers. The leak rates of urea and creatinine across LLC‐PK 1 cell‐attached polysulfone membrane modules (membrane areas: 56 cm 2 and 4000 cm 2 ) were investigated. Via conversion from 56 m 2 to 1 m 2 hollow fiber modules with LLC‐PK 1 cells for 24 h, the transport rates of H 2 O, glucose and Na + were, respectively, 40, 65 and 35% of the target transported amounts from 6 L/day of filtrate. The rates are expected to approach 100% when 4–5 g/dL of albumin is added to the basal portion of the medium since the results were obtained without the addition of albumin for colloidal osmotic pressure.