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Effects of Customized Bicarbonate Buffered Solutions for Continuous Renal Replacement Therapies on Polymorphonuclear Leukocytes Function and Viability
Author(s) -
Maccariello Elizabeth,
Dalboni Maria A.,
Draibe Sérgio A.,
Rocha Eduardo,
Santos Oscar Fernando Pavão dos,
Santos Bento,
Cendoroglo Miguel
Publication year - 2004
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2004.07284.x
Subject(s) - bicarbonate , calcium , chemistry , sodium bicarbonate , incubation , phagocytosis , flow cytometry , chromatography , biochemistry , medicine , immunology , organic chemistry
It has previously been shown that the mixture of bicarbonate and calcium in the solutions used for continuous renal replacement therapy led to crystalization and significant changes in calcium concentration and pH. The aim of this study was to investigate the impact of bicarbonate/calcium and lactate/calcium solutions for Continuous Renal Replacement Therapies (CRRT) on the viability and function of polymorphonuclear cells (PMN). We tested four customized bicarbonate buffered solutions: single bag (bicarbonate and calcium mixed 24 h before testing), double bag (mixed immediately before testing), filtered single bag and double bag solutions, and a commercial lactate buffered solution. Blood from 6 volunteers was incubated with the solutions for 30 min followed by PMN isolation. After overnight incubation, viability, phagocytosis, and peroxide production by PMN were determined by flow cytometry. There was no difference between the test solutions with respect to PMN viability and function. Therefore, the presence of microcrystals and the consequent changes in electrolyte concentrations do not seem to impair PMN function.