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Phenotypic Alterations in Circulating Monocytes Induced by Open Heart Surgery Using Heparinized and Nonheparinized Cardiopulmonary Bypass Systems
Author(s) -
Ljunghusen Olof,
Cederholm Ingemar,
Lundahl Joachim,
Nilsson Bo,
Olin Christian,
Sjogren Florence,
Stendahl Olle
Publication year - 1997
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1997.tb00448.x
Subject(s) - cardiopulmonary bypass , monocyte , integrin alpha m , leukocytosis , flow cytometry , immunology , complement system , medicine , phenotype , granulocyte , biology , antibody , biochemistry , gene
In this study of 31 patients with coronary bypass surgery, we used flow cytometry to compare heparin‐coated and noncoated cardiopulmonary bypass systems on leukocyte activation. We found significant differences between the groups during bypass, with activation of the complement system, measured as elevated levels of C3a desArg, upregulation of granulocyte β 2 integrin (CD11b). and a loss of circulating monocytes when noncoated systems were used. In both groups an early increase in the monocyte cell surface CD62L expression was obvious while the percentage of human leukocyte antigen (HLA)‐DR positive monocytes did not alter. The morning after the operation, leukocytosis was present. together with a highly significant reduction in the monocyte expression of CDllb and HLA‐DR, indicating the recruitment to the peripheral blood of cells with altered phenotypes. This alteration in phenotype on potent inflammatory cells may be one part of the impaired function of the immunological system reported after major surgery.