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Influence of Blood Flow on Adsorption of β 2 ‐Microglobulin onto AN69 Membrane
Author(s) -
Kandus Aljoša,
Malovrh Marko,
Bren Andrej F.
Publication year - 1997
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1997.tb00249.x
Subject(s) - chemistry , adsorption , beta 2 microglobulin , membrane , chromatography , dialysis tubing , ultrafiltration (renal) , polyacrylonitrile , dialysis , blood flow , hemodialysis , medicine , biochemistry , polymer , organic chemistry
Adsorption onto the dialyzer membrane is a contributing factor to the elimination of β 2 ‐microglobulin (β 2 M) from the sera of uremic patients. The purpose of this prospective study was to ascertain the influence of the blood flow rate on adsorption of β 2 M onto the polyacrylonitrile (AN69) hollow‐fiber dialyzer membrane in 8 pa tients during regular hemodialysis (HD). Blood first passed through a low‐flux polysulfone dialyzer and then through an AN69 dialyzer, which was not in contact with the dialysis fluid. During the investigation period (first hour of the HD session), the blood flow rate was 100 ml/min (first part of the study), 200 mumin (second part of the study), and 300 ml/min (third part of the study). Ultrafiltration was not performed during the investigation period. At the start of the HD sessions, the serum concentration of β 2 M in the afferent blood line did not differ significantly among the 3 parts of the study. Serum β 2 M was measured in samples taken from the afferent and efferent blood lines of the AN69 dialyzer at 5,10, 15, 30, 45, and 60 min. The serum β 2 M concentration decreased significantly in blood that had passed through the AN69 dialyzer. This decrease, indicating membrane adsorption, was maximal during the first part and minimal during the third part of study. The decrease in the contact time between the blood and the AN69 could be the underlying cause. The calculated quantities of β 2 M adsorbed onto the AN69 membrane (44.2 ± 10.2, 43.2 ± 12.1, and 42.6 ± 17.3 mg) did not differ significantly among the 3 parts of the study. These results suggest that an increase in blood flow rate from 100 to 300 ml/min did not significantly affect the quantity of β 2 M adsorbed onto the AN69 membrane.