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Long‐term Performance of Albumin Secretion of Hepatocytes Cultured in a Packed‐bed Reactor Utilizing Porous Resin
Author(s) -
Miyoshi Hirotoshi,
Yanagi Kennichi,
Fukuda Hideki,
Ohshima Norio
Publication year - 1996
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1996.tb04545.x
Subject(s) - monolayer , albumin , perfusion , chromatography , packed bed , secretion , chemistry , serum albumin , in vitro , chemical engineering , materials science , biochemistry , medicine , engineering
A packed‐bed reactor utilizing the porous polymer, polyvinyl formal (PVF) resin, as a supporting material was applied for the long‐term culture of rat hepatocytes. Perfusion cultures using this reactor, as well as monolayer cultures, were performed for up to 1 week under 3 different media conditions. When serum‐free or epidermal growth factor‐containing medium was used, albumin secretion rates showed constant decreases in both the perfusion and the monolayer cultures, and approximately 10% of the activity exhibited on Day 1 was preserved at the end of the cultures. In contrast, the hepatocytes supplemented with serum‐containing medium exhibited stable ability of albumin secretion throughout the culture period under the perfusion culture condition using PVF resin. From the microscopic observation, the immobilized hepatocytes in this medium revealed round shapes, and a cluster of cell aggregates was scarcely seen.