Premium
Hemodialysis with Cellulose Membranes Primes the Neutrophil Oxidative Burst
Author(s) -
Ward Richard A.,
McLeish Kenneth R.
Publication year - 1995
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1995.tb02431.x
Subject(s) - respiratory burst , neutropenia , hemodialysis , dialysis , membrane , chemistry , medicine , priming (agriculture) , granulocyte , immunology , biochemistry , biology , toxicity , germination , botany
Hemodialysis with cellulose membranes causes a complement‐mediated neutropenia. Changes in neutrophil function have also been reported; however, it is unclear if these changes indicate a direct effect of the membrane on neutrophils or if they are a consequence of the neutropenia. We tested the hypothesis that neutrophil oxidative burst activity is enhanced during dialysis with cellulose membranes. Resting and Staphylococcus aureus ‐stimulated H 2 O 2 production were determined predialysis and in blood entering and leaving the dialyzer during the first 30 min of dialysis and in blood leaving the membrane module in a single‐pass on‐line model of hemodialysis. Resting H 2 O 2 production increased slightly but significantly during the first 30 min of dialysis. Transit of neutrophils through the dialyzer caused a marked increase in stimulated H 2 O 2 production, indicating priming of the oxidative burst. However, priming was limited to the first 5 min of dialysis before the onset of neutropenia. In contrast, stimulation and priming of H 2 O 2 production persisted throughout 30 min of single‐pass on‐line perfusion. These results indicate that cellulose membranes both stimulate and prime neutrophil oxidative burst activity but that these effects are partially obscured by neutropenia.