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High Cell‐Density Culture System of Hepatocytes Entrapped in a Three‐Dimensional Hollow Fiber Module with Collagen Gel
Author(s) -
Takeshita Kazuyoshi,
Ishibashi Haruaki,
Suzuki Masayuki,
Yamamoto Takumi,
Akaike Toshihiro,
Kodama Masashi
Publication year - 1995
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1995.tb02310.x
Subject(s) - hepatocyte , secretion , bioartificial liver device , monolayer , cell culture , chemistry , albumin , biomedical engineering , cell , chromatography , biochemistry , microbiology and biotechnology , in vitro , biology , medicine , genetics
Abstract: A compact three‐dimensional (3D) module is needed for hepatocyte culture in order to develop an effective hybrid artificial liver system that can retain hepa‐tocellular structure and differentiated functions. We treated the 3D module with collagen gel to entrap rat hepatocytes. This method yielded a high hepatocellular density (2 times 10 7 cells/ml) over a period of 14 days and maintained the secretion of albumin and ureogenesis at the same level as the control monolayer method. The ammonia removal remained at 43% of the Day 0 value over 8 days of perfusion. Our data show that this approach may be useful for liver support therapy in an ex‐tracorporeal circuit.