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In Vitro and In Vivo Evaluation of Protamine–Heparin Membrane for Microencapsulation of Rat Langerhans Islets
Author(s) -
Tatarkiewicz Krystyna,
Sitarek Elzbieta,
Fiedor Piotr,
Sabat Marek,
Orlowski Tadeusz
Publication year - 1994
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1994.tb03311.x
Subject(s) - protamine , in vivo , heparin , islet , in vitro , insulin , transplantation , pancreatic islets , chemistry , endocrinology , medicine , renal capsule , secretion , membrane , capsule , biology , biochemistry , microbiology and biotechnology , botany
Rat pancreatic islets were microencapsulated with multilayer protamine–heparin (PH) membrane. Basal and stimulatory insulin secretion of microencapsulated islets was similar to the controlled free islets in vitro. During the long–term culture (up to 2 weeks) mean insulin release of encapsulated islets did not significantly differ from the mean of free ones (the ratio of mentioned means was 54–167%). Empty PH microcapsules transplanted into Wistar rats intraperitoneally and under the kidney capsule were generally harmless up to 4 months. In only a few cases traces of fibrotic tissue around capsules entrapped in the omentum were found. No damage of microcapsules structure was observed. The worst results were obtained in the instance of retroperitoneal transplantation. We conclude, therefore, that PH membrane was proved to be highly biocompatible, nontoxic for islets, and did not impair viability and glucose–dependent insulin secretion of Langerhans islets in in vitro culture.