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Comparison of Pig Hepatocyte Isolation Using Intraoperative Perfusion without Warm Ischemia and Isolation of Cells from Abattoir Organs after Warm Ischemia
Author(s) -
Gerlach Jörg,
Klöppel Karolin,
Schön Michael R.,
Brombacher Jochen,
Courtney James M.,
Unger Juliane,
Neuhaus Peter
Publication year - 1993
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1993.tb00409.x
Subject(s) - trypan blue , perfusion , hepatocyte , ischemia , viability assay , medicine , andrology , biology , pathology , cell , biochemistry , in vitro
Enzymatic hepatocyte isolation using warm ischemic pig livers from an abattoir was compared with isolation using in situ perfused organs. Using organs from animals of 30 kg body mass (BM), the intraoperative perfusion showed superior results. The use of livers from abattoir pigs of 40–50 kg BM after warm ischemia resulted in a lower yield of hepatocytes and in high rates of injured cells. The mean yield in the intraoperative perfusion group was 68 ± 11% (wet weight), the maximum yield in the abattoir organ group was 58%. The mean viability in the intraoperative perfusion group was 65 ± 14% (trypan blue) compared with a maximum viability of 39% in the abattoir liver group. Additional purification by density gradient centrifugation improved the viability of the abattoir liver group to a mean of 95% (trypan blue). The use of pig livers from large abattoir animals required additional purification steps to improve viability since the cell yield is considerably lower than with intraoperative organ perfusion. In general, hepatocyte isolation from abattoir organs is not recommended.