Premium
Effects of Pyridoxalated Hemoglobin Polyoxyethylene Conjugate and Stroma Free Hemoglobin on Pulmonary Vascular Responsiveness to Vasoactive Substances in Isolated Perfused Rat Lungs
Author(s) -
Gotoh Keiji,
Matsumura Hiroaki,
Morioka Tohru,
Nishi Katsuhide
Publication year - 1992
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1992.tb00556.x
Subject(s) - perfusion , hemoglobin , acetylcholine , chemistry , vasoactive , bradykinin , lung , angiotensin ii , medicine , blood pressure , endocrinology , biochemistry , receptor
We examined the effects of pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) and stroma free hemoglobin (SFH) on vascular responsiveness to various vasoactive substances in isolated perfused rat lungs. The lungs isolated from rats were perfused with 6% PHP, 6% SFH, or 6% hydroxyethylstarch (HES) solution, and the effects of intrapulmonary arterial injection of norepineph‐rine (NE), angiotensin II (ANG‐II), acetylcholine (ACh), and nitroglycerin (NG) were examined by measuring per‐fusion pressure. NE and ANG‐II produced a dose‐dependent increase in perfusion pressure in all groups. The NE response in the PHP‐ or SFH‐perfused group was significantly larger than that in the HES‐perfused one. ACh decreased perfusion pressure in both PHP‐ and HES‐perfused groups but increased perfusion pressure in the SFH‐perfused group. NG decreased perfusion pressure in all groups. Present results indicate that pulmonary arterial responses to endothelium‐derived relaxing factor (EDRF) induced by ACh would not be affected in the presence of PHP.