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Effects of Temperature on Phagocytosis of Human and Calf Polymorphonuclear Leukocytes
Author(s) -
Utoh Junichi,
Harasaki Hiroaki
Publication year - 1992
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1992.tb00535.x
Subject(s) - phagocytosis , opsonin , in vitro , chemistry , granulocyte , andrology , microbiology and biotechnology , immunology , pharmacology , endocrinology , biology , medicine , biochemistry
The in vitro effect of temperature on the phago‐cytic function of human and calf polymorphonuclear leukocytes (PMN) was studied. To observe the PMN phago‐cytic function (PPF) at various temperatures, PMN from healthy human and calf donors were incubated with serum‐opsonized fluorescent latex particles (diameter 1.66 γm) at 25, 37, 40, 42, 44, or 46°C for 1 h and then observed for their phagocytic activity by fluorescent microscopy. At 25, 40, and 42°C, human PPF was not significantly different from that at 37°C (87%, 89%, and 80% vs. 93%). At 44°C, PPF was noticeably depressed (19%, p < 0.05) when compared to that at 37°C. Next, to determine the critical temperature and duration of exposure that would irreversibly damage PPF, PMN were preincubated at 42, 44, or 46°C for 5–30 min before being subjected to a standard phagocytosis assay at 37°C. The human PPF was significantly depressed after 30 rnin at 44°C (33%, p < 0.05) or 10 min at 46°C (30%, p < 0.05). In conclusion, neither human nor calf PPF was significantly altered at and below 42°C. In contrast, the PPF was irreversibly and time‐dependently damaged when incubated at and above 44°C.