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Vascular Responsiveness to Various Vasoactive Substances After Exchange Transfusion with Pyridoxalated Hemoglobin Polyoxyethylene Conjugate (PHP) Solution in Anesthetized Rats
Author(s) -
Kida Yoshitoshi,
Iwata Seinosuke,
Gyoutoku Yuichi,
Aikou Akira,
Yamakawa Takashi,
Nishi Katsuhide
Publication year - 1991
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1991.tb00753.x
Subject(s) - chemistry , perfusion , norepinephrine , hemoglobin , acetylcholine , angiotensin ii , exchange transfusion , blood pressure , anesthesia , blood substitute , medicine , endocrinology , pharmacology , dopamine , biochemistry
Vascular responsiveness to various vasoactive substances after exchange transfusion with a modified hemoglobin (pyridoxalated hemoglobin polyoxyethylene conjugate; PHP) was examined in anesthetized rats. The hindlimb region of the rat was perfused through the abdominal aorta with autologous blood at a constant flow rate, using a perfusion pump. Perfusion pressure (PP), systemic blood pressure (SBP), and heart rate were measured. Exchange transfusion with a 6% PHP solution (20 ml/kg) was performed and effects of intra–arterial injections of norepinephrine (NE), angiotensin–II (A–II), serotonin (5–HT), acetylcholine (ACh), and nitroglycerin (NG) on PP and SBP were examined. The NE and A–II induced a dose–dependent increase in PP, while ACh and NG induced a decrease in PP. There was no difference in vascular responsiveness to ACh, NG, A–II, and 5–HT before and after exchange transfusion with PHP solution, but NE–induced responses were augmented after exchange transfusion with PHP. Results suggest that the presence of PHP moiety in circulation would not alter vascular responsiveness to ACh, NG, A–II, and 5–HT in a significant manner.