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The Heterogeneity of Protein/Surface Interactions and Structural Alterations of Adsorbed Albumin and Immunoglobulin G
Author(s) -
Kulik Eduard A.,
Kalinin Igor D.,
Sevastianov Victor I.
Publication year - 1991
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1991.tb00748.x
Subject(s) - adsorption , kinetics , chemistry , human serum albumin , kinetic energy , logarithm , molecule , chemical physics , thermodynamics , protein adsorption , activation energy , biophysics , chromatography , organic chemistry , physics , mathematical analysis , mathematics , quantum mechanics , biology
The theoretical model is developed for the reversible and irreversible protein adsorption in kinetic regime by assuming the continuous energetical heterogeneity for protein/surface interaction and the possibility of structural alterations of adsorbed molecules. The simplest rectangular distributions of adsorption centers in energy of activation are used to explain the logarithmic kinetics of IgG and human serum albumin (HSA) adsorption on a quartz surface. To explain the Freindlich character of HSA adsorption onto a precoated surface, the exponential distributions of adsorption centers in energy of activation are used. A competitive analysis of some of the approaches allowed for the energetical heterogeneity of protein/surface interaction is made. The possibility of lateral electrostatic repulsion to form the logarithmic kinetics of HSA adsorption is checked experimentally. The influence of the temperature on HSA adsorption onto quartz is discussed also.