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The Interaction of Heparinized Biomaterials with Human Serum, Albumin, Fibrinogen, Antithrombin III, and Platelets
Author(s) -
Nemets Eugene A.,
Sevastianov Victor I.
Publication year - 1991
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1991.tb00747.x
Subject(s) - antithrombin , fibrinogen , chemistry , adsorption , platelet , adhesion , platelet adhesiveness , coagulation , albumin , human serum albumin , copolymer , polymer chemistry , blood proteins , protein adsorption , heparin , polymer , chromatography , biochemistry , organic chemistry , platelet aggregation , immunology , medicine , biology
The influence of the method of heparin (HEP) immobilization on human serum albumin (HSA), fibrinogen (FG), and antithrombin III (AT–III) adsorption, platelet adhesion, and activation on the surface of polyvinylchloride, polyurethane Vitur, and a copolymer of styrene and divinylbenzene was measured. The negative correlation between the degree of irreversibility of plasma protein adsorption and the amount of adsorbed AT–III for HEP, immobilized onto the polymer surface passivated with HSA, FG, and plasma was found. The same negative correlation was observed between the amount of AT–III adsorbed on these systems and the number of adhered platelets. Schemes of the interaction of surface bound–HEP with AT–III, including the influence of an irreversibly adsorbed protein layer and adhered platelets, have been proposed. The essential role of AT–III in heparinized biomaterials/platelet interaction has been shown. A new method of combined immobilization of HEP and platelet adhesion inhibitor has been elaborted on.