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Effects of Pyridoxalated Hemoglobin Polyoxyethylene Conjugate and Stroma‐Free Hemoglobin on Cardiac Function in Isolated Rat Heart
Author(s) -
Yamakawa Takashi,
Miyauchi Yoshimasa,
Nishi Katsuhide
Publication year - 1990
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1990.tb02959.x
Subject(s) - hemoglobin , conjugate , stroma , blood substitute , cardiac function curve , chemistry , medicine , cardiology , biochemistry , heart failure , mathematics , mathematical analysis , immunohistochemistry
Effects of a newly introduced modified hemoglobin, pyridoxalated hemoglobin polyoxyethylene conjugate (PHP), and stroma‐free hemoglobin (SFH) on cardiac rhythm and contractility were examined in isolated rat hearts. Hearts were perfused with HEPES‐buffered Krebs solution containing 6% PHP, 6% SFH, or 6% hydroxy ethylstarch (HES). Arrhythmia was not observed in PHP‐perfused hearts, but marked sinus bradycardia occurred in SFH‐perfused hearts. Ventricular contractility assessed by left ventricular pressure and pressure‐volume relationship in both PHP and SFH‐perfused hearts were well maintained during perfusion. There was no significant difference to the coronary flow resistance between hearts perfused with PHP and HES solutions, indicating that PHP has little vasoactive effect. The flow resistance in SFH‐perfused hearts decreased significantly when compared with other groups. Results demonstrate that PHP solution can preserve cardiac function and have no toxic effects in isolated rat hearts.