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Changes in Neutrophil Oxidative Potential in Patients Undergoing Cardiopulmonary Bypass with Polypropylene Hollow Fiber Oxygenators
Author(s) -
Roccatello Dario,
Formica Marco,
Cavalli Guido,
Amprimo Maria C.,
Pignatelli Maria G.,
Costa Paolo,
Paulis Ruggero,
Quattrocchio Giacomo,
Molino Andrea,
Giordano Gianbeppe
Publication year - 1990
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1990.tb01594.x
Subject(s) - cardiopulmonary bypass , zymosan , respiratory burst , chemiluminescence , chemistry , medicine , opsonin , phagocyte , phagocytosis , anesthesia , immunology , biochemistry , chromatography , in vitro
Neutrophil oxidative metabolism, C3d and β2 microglobulin levels, were assessed in nine consecutive patients undergoing cardiopulmonary bypass surgery with polypropylene hollow fiber oxygenators for open cardiac operations. Generation of oxygen free radicals by neutrophils was measured as luminol‐enhanced chemiluminescence after stimulation with opsonized Zymosan and phorbol myristate acetate. A significant increase in light emission was detected by using both of the chemiluminescence stimulators. Moreover, a remarkable and significant increase in C3d levels was found already at 10 min. Conversely minimal changes in levels of p2 microglobulin were detected during cardiopulmonary bypass surgery. These data suggest that the impact of the patient blood with the foreign surface of cardiopulmonary bypass results in activation of phagocyte cells with increased potential in oxygen consumption. These effects could be partially complement‐mediated.