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Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding
Author(s) -
Vohra R. K.,
Thompson G. J. L.,
Sharma H.,
Carr H. M. H.,
Walker M. G.
Publication year - 1990
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1990.tb01590.x
Subject(s) - fibronectin , chemistry , polytetrafluoroethylene , coating , incubation , seeding , matrix (chemical analysis) , endothelial stem cell , andrology , biophysics , chromatography , extracellular matrix , biochemistry , in vitro , medicine , biology , organic chemistry , agronomy
Although fibronectin's role as a matrix to improve endothelial seeding has been demonstrated by other workers, the optimum concentration for use has never been described. Attachment of fibronectin to expanded polytetrafluoroethylene (ePTFE) was measured, using 125 I‐radiolabeled protein, at different concentrations and for different time periods. The absolute amount of fibronectin bound to the graft increased with the concentrations used in coating (p < 0.001) and also with time (p < 0.01); e.g., at 50 μg/ml, 90 min of incubation produced a molecular attachment of 4.0 × 10 11 /cm 2 of graft. However, its percentage attachment decreased with a rise in concentration (p < 0.001). After an initial loss of 22% in 30 min, the fibronectin‐graft bond was found to be stable when exposed to a shear stress produced by flow at 200 ml/min. No significant difference in the cell adherence could be found in grafts coated with fibronectin concentrations of 50, 150, and 250 μg/ml, although it was significantly less at 10 and 25 μg/ml (p < 0.05).