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Eicosanoid Release as Laboratory Indicator of Biocompatibility
Author(s) -
Mahiout A.,
Jörres A.,
Schultze G.,
Meinhold H.,
Kessel M.
Publication year - 1989
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1989.tb02872.x
Subject(s) - thromboxane b2 , chemistry , eicosanoid , membrane , whole blood , thromboxane , incubation , perfusion , in vivo , autacoid , biocompatibility , extracorporeal circulation , blood cell , in vitro , pharmacology , platelet , biochemistry , medicine , arachidonic acid , biology , receptor , microbiology and biotechnology , organic chemistry , enzyme
Biocompatibility evaluation of extracorporeal devices requires the establishment of sensitive indicators of blood cells/surface interactions. Among others, arachidonic acid derivatives, such as prostaglandins and thromboxanes, play an important role in the cell control systems. Hence, the release of eicosanoids during blood exposure to dialyzer membranes was investigated. Experiments included in vitro incubation of human blood with flat membranes (FM), as well as ex vivo perfusion of hollow fiber membranes (HFM) with blood from healthy volunteers in single‐pass fashion. In both models, a significant release of prostaglandin E 2 (PGE 2 ) and thromboxane B 2 (TXB 2 ) was detected. In addition, the amount of eicosanoid release depended on the type of membrane tested. After a 10‐min FM incubation with fresh blood, plasma concentrations of TXB 2 and PGE 2 were pronounced by polycarbonate when compared to Cuprophan and poly aery lonitrile. During 10 min of open loop perfusion of HFM, polymethylmethacrylate was the most active biomaterial, whereas the reactivity of Cuprophan was significantly lower. Among HFM, Hemophan was by far the less active. These results indicate that the release of eicosanoids represents a sensitive parameter of blood cells/membrane reactivity. Thus, the question arises as to whether or not the extracorporeal process of cyclooxy‐genase activity could contribute to the clinical side effects of chronical hemodialysis.