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The Effects of PGI 2 Analog (OP‐41483) on Perfused Porcine Liver
Author(s) -
Kimoto Michio,
Shimahara Yasuyuki,
Ikai Iwao,
Wakashiro Shigetaro,
Ozaki Nobuhiro,
Tatsumi Yoshio,
Tanaka Akira,
Kamiyama Yasuo,
Yamaoka Yoshio,
Ozawa Kazue
Publication year - 1989
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1989.tb02864.x
Subject(s) - perfusion , chemistry , ketone bodies , nicotinamide , nad+ kinase , metabolism , endocrinology , medicine , microgram , biochemistry , in vitro , enzyme
The effects of a prostacyclin analog OP‐41483 on energy metabolism were studied in an isolated porcine liver perfused with human blood for 8 h. OP‐41483 was administered intravenously at a rate of 0.3 μg/kg/min during the procurement and into the perfusate at a rate of 1.0 μg/min during perfusion. Acetoacetate, ß‐hydroxy‐butyrate, lactate, and pyruvate were measured before perfusion and at 1,2, 3, 5, and 8 h after perfusion, from which values the ketone body ratio (acetoacetate/ß‐hydroxybutyrate, KBR), reflecting the redox state of liver mitochondria, was calculated. In the OP‐41483 group, KBR increased rapidly from 0.34 to 0.95, 1.61, 1.51, 2.35, and 2.04, and lactate decreased rapidly from 9.81 to 6.30, 4.51, 3.22, 2.39, and 1.33 mmol/L at the respective hours after perfusion. There were significant differences after 3 h of perfusion as compared with the control group (p<0.05). These results suggest that administration of OP‐41483 causes an increase in mitochondrial NAD+/NADH ratio (oxidized and reduced forms of free nicotinamide‐adenine dinucleotides), leading to an enhancement of the metabolic capacity of the perfused liver.