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Influence of High and Low Wall Shear Rates on the Inhibition of Factor Xa and Thrombin at Surfaces Coated with Immobilized Heparin
Author(s) -
Arnander Claes,
Pasche Boris,
Kodama Kenji,
Rasmuson Anders,
Olsson Per
Publication year - 1989
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1989.tb01573.x
Subject(s) - thrombin , heparin , chemistry , protease , antithrombin , serine protease , shear (geology) , chromatography , shear rate , biophysics , biochemistry , enzyme , materials science , composite material , medicine , biology , platelet , viscosity
The thromboresistant function of a surface with end‐point attached heparin is based upon interaction among the immobilized heparin, antithrombin, and at least factor Xa or thrombin. Heparinized arteriovenous shunts were implanted in dogs. By compressing a segment of the shunt, high and low wall shear rate regions were obtained in each shunt. After removal, the tubings were tested for their factor Xa and thrombin inhibitory capacity. It was found that on a molar basis, the factor Xa and thrombin inhibitory capacity were similar in low wall shear rate segments. In high wall shear rate segments, the thrombin inhibitory capacity was decreased, thus indicating that the AT‐mediated inhibition of the serine protease is dependent on the wall shear rate.