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Molecular Design of Materials for Cell Separation
Author(s) -
Kataoka Kazunori
Publication year - 1988
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1988.tb02816.x
Subject(s) - copolymer , desorption , yield (engineering) , adsorption , azobenzene , methacrylate , ionic bonding , chemical engineering , chromatography , chemistry , materials science , elution , polymer chemistry , molecule , organic chemistry , polymer , ion , engineering , metallurgy
: There has been a strong demand in biomedical sciences to isolate viable cell populations with high yield and purity. An important facet of this work was to develop new polymeric adsorbent for the separation of lymphocyte subpopulations. Based on our strategy of separating cells through their differential ionic affinity toward multiphase‐structured adsorbent with ionically derivatized microdomains, a series of poly(2‐hydroxyethyl methacrylate)/polyamine graft copolymers (HA copolymers) was prepared. HA copolymer columns were found to show specific adsorption affinity toward B lymphocytes, and allows for separation of B and T lymphocytes in high yield and purity with a short operating time. Separation mechanism involved in the resolution of B and T lymphocytes by HA copolymer column is discussed in this paper. Further, photo‐induced desorption of cells from the adsorbent derivatized with photo‐responsive functional group (azobenzene group) was demonstrated to emphasize the feasibility of photo‐regulated chromatography as a novel tool in cell separation technology.